Journal: Archives of Toxicology
Article Title: Standardization and optimization of the hiPSC-based PluriLum assay for detection of embryonic and developmental toxicants
doi: 10.1007/s00204-024-03870-8
Figure Lengend Snippet: Comparison of effects of exposure to all- trans (a t RA) and 13- cis retinoic acid (13- cis RA) in the PluriLum assay upon assay completion on D6 and D7. A The effects of exposure to a t RA on average NKX2.5- derived luminescence. B Visualization of contractility as an effect of exposure to a t RA. C The effects of exposure to 13- cis RA on average NKX2.5- derived luminescence. D Visualization of contractility as an effect of exposure to 13- cis RA
Article Snippet: L-Ascorbic acid 2-phosphate trisodium salt (Asc), sodium selenite, human transferrin, all- trans (a t RA; CAS number 302-79-4), and 13- cis retinoic acid (13- cis RA; CAS number 4759-48-2) were purchased from Merck KGaA (Darmstadt, Germany).
Techniques: Comparison, Derivative Assay